課程資訊
課程名稱
生物電子顯微鏡技術學
Biological Electron Microscopy 
開課學期
104-2 
授課對象
生命科學院  生態學與演化生物學研究所  
授課教師
陳香君 
課號
EEB5043 
課程識別碼
B44 U1550 
班次
 
學分
全/半年
半年 
必/選修
選修 
上課時間
星期二2,3(9:10~11:10) 
上課地點
生科419 
備註
初選不開放。正課為23,1、4、@堂為實習,須先與老師聯絡。
總人數上限:12人 
Ceiba 課程網頁
http://ceiba.ntu.edu.tw/1042BioEM_EEB5043 
課程簡介影片
 
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課程概述

1) Introduction Orientation
2) Preparation for TEM I Sample Fixation & Embedding, Block Trimming
3) Specimen Preparation for TEM II Supporting Film & Glass Knife Making
4) Ultramicrotomy Ultramicrotomy I
5) Section Staining Ultramicrotomy II
6) Principle of EM Section Staining
7) Structure and Operation of TEM Structure and Operation of TEM
8) Cytochemistry & Immunomicroscopy Negative Staining, Shadow-Casting Technique & Lab
9) Scanning Electron Microscopy Analytical Electron Microscopy & Light Micrography
10) Cryo-EM Techniques Freeze-Etching Technique
11) Mid. Examination Cytochemistry & Immunomicroscopy
12) SEM Sample Preparation Darkroom Technique
13) Image Presentation and Analysis Scanning Analytical Electron Microscopy
14) Fluorescent Microscopy Confocal Microscopy & Campus EM Centers
15) Perspectives of Electron Microscopy Academia Sinica EM Centers
16) Presentation of EM-Lab Data Individual assistance to student projects
17) Final Examination
18) Hand in Report
 

課程目標
熟悉各種生物電顯相關技術與原理 
課程要求
 
預期每週課後學習時數
 
Office Hours
 
指定閱讀
 
參考書目
 
評量方式
(僅供參考)
   
課程進度
週次
日期
單元主題
第1週
02/23  Lec: Introduction & Specimen Preparation for TEM
Lab: Orientation 
第2週
03/01  Lec: Ultramicrotomy
Lab: Sample Fixation, Embedding and Block Trimming 
第3週
03/08  Lec: Section staining & Negative staining
Lab: Supporting film & Glass knife making 
第4週
03/15  Lec: Principle of EM
Lab: Ultramicrotomy I 
第5週
03/22  Lec: Structure and Operation of TEM
Lab: Ultramicrotomy II  
第6週
03/29  Lec: Midexam I
Lab: Structure and Operation of TEM; Section staining & Negative staining 
第7週
04/05  Holiday 
第8週
04/12  Lec: Scanning electron microscopy
Lab: Presentation of LM images 
第9週
04/19  Lec: Analytical electron microscopy
Lab: SEM Preparation 
第10週
04/26  Lec: Cryo-Electron Microscopy
Lab: SEM Observation 
第11週
05/03  Lec: Cytochemistry & Immuno-microscopy
Lab: Immuno-electron microscopy 
第12週
05/10  Lec: Image Observation & Interpretation
Lab: Individual assistance to student projects 
第13週
05/17  Lec: Image Processing and Analysis
Lab: TM-3000 (理學院電顯室: 林錦燕小姐) 
第14週
05/24  Lec: Midexam II
Lab: Analytical Electron Microscopy (材料系:陳學人先生)
 
第15週
05/31  Lec: Perspectives of Electron Microscopy
Lab: Individual assistance to student projects 
第16週
06/07  Academic Sinica EM Centers  
第17週
06/14  Presentation of EM-Lab Data  
第18週
06/21  Hand in Report